Flagella and Cell Body Fluorescent Staining

Flagella were stained with the fluorescent dye Alexa 488 C₅ maleimide (Thermo Fisher Scientific). The staining was carried out as published before in Hintsche et al. (19 (link)). For agar experiments, we additionally stained the cell body with 10 μL FM 4-64 (Thermo Fisher Scientific; 1 μg/μL in dimethyl sulfoxide [DMSO]). It was added before the last washing step. Previous experiments showed that the double staining does not affect the swimming modes.

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Pfeifer V., Beier S., Alirezaeizanjani Z, & Beta C. (2022). Role of the Two Flagellar Stators in Swimming Motility of Pseudomonas putida. mBio, 13(6), e02182-22.

Publication 2022

Alexa 488 C5 maleimide FM 4-64

Agar Cell body Dimethyl sulfoxide Flagella Fluorescent dye Fm 4 64 Maleimide

Corresponding Organization : University of Potsdam

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Variable analysis

independent variables

Staining of flagella with the fluorescent dye Alexa 488 C5 maleimide
Staining of cell body with FM 4-64 dye

dependent variables

Swimming modes

control variables

Previous experiments showed that the double staining does not affect the swimming modes

controls

No positive or negative controls were explicitly mentioned in the provided information.

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