Immunohistochemical Detection of PPRV Antigen

The paraffin sections of different organs from both PPRV-infected and control goats collected at 7, 10, 14, and 18 dpi were analyzed by immunohistochemistry for the localization of PPRV antigen. A monoclonal antibody against N protein of PPR virus (clone 38-4), received as a gift from IAEA, Vienna, Austria, was used as the primary antibody (12 (link), 31 (link)). A commercial immunoperoxidase (IP) detection system, Dako EnVision® Dual Link System-HRP (DAB+) kit (Agilent Technologies, Santa Clara, CA, USA) was used to detect the bound primary antibodies. The paraffin sections were mounted on poly-l-lysine coated slides (Sigma, St. Louis, MO, USA). The IP staining was performed as described in the kit literature. With each group of staining, sections from the uninfected control group were also stained similarly to serve as the negative control.