Multiplex Immunohistochemistry for Tissue Analysis

Immunohistochemistry (IHC) staining was performed in tissue sections as described previously (22 (link)). Sections were incubated with goat anti-NEK7 (ab166776, Abcam, Cambridge, MA, USA) and anti-GSDMD (ab219800, Abcam) overnight at 4°C and stained with a second antibody by using an SP-POD Kit according to the manufacturer’s instructions (#SP0041, Solarbio, Beijing, China). Multiplexed IHC (mIHC) was performed using a multiple fluorescent immunohistochemical staining kit (abs50012, Absin, Shanghai, China) according to the manufacturer’s instructions (23 (link)). Sections were observed using an optical microscope (BX53, Olympus, Tokyo, Japan).

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Yan Z., Da Q., Li Z., Lin Q., Yi J., Su Y., Yu G., Ren Q., Liu X., Lin Z., Qu J., Yin W, & Liu J. (2022). Inhibition of NEK7 Suppressed Hepatocellular Carcinoma Progression by Mediating Cancer Cell Pyroptosis. Frontiers in Oncology, 12, 812655.

Publication 2022

ab166776 ab219800 SP-POD Kit abs50012

Antibody Goat Immunohistochemistry Optical microscope Tissue

Corresponding Organization :

Other organizations : Peking University Shenzhen Hospital, University of Hong Kong - Shenzhen Hospital

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Variable analysis

independent variables

Incubation of sections with goat anti-NEK7 and anti-GSDMD antibodies overnight at 4°C

dependent variables

Immunohistochemistry (IHC) staining and multiplexed IHC (mIHC) of tissue sections

control variables

Sections were stained with a second antibody using an SP-POD Kit according to the manufacturer's instructions
Multiplexed IHC (mIHC) was performed using a multiple fluorescent immunohistochemical staining kit according to the manufacturer's instructions
Sections were observed using an optical microscope (BX53, Olympus, Tokyo, Japan)

controls

No positive or negative controls were explicitly mentioned

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