To measure the relative expression of TLR7, TLR8, and MyD88, RNA was extracted from 5 × 105 cells using AxyPrep Multisource RNA Miniprep Kit (Axygen) and measured by Nano-drop. An equal amount of RNA was reverse-transcribed into cDNA through the iScript cDNA Synthesis Kit (Bio-red). Samples were quantified by PCR with SYBR Green (Applied Biosystem). All specific primers used for the analysis were designed by qPrimerDB (Lu et al., 2018 (link)), and the sequences were shown as follows:
To measure the HIV viral load, viral RNA from supernatants was extracted by MagaBio Plus Viral DNA / RNA Purification Kit (BIOER). The reverse-transcription and analysis of RNA copies were performed using the HIV-1 Real-time PCR detection kit (BIOER).
To measure the HIV viral load, viral RNA from supernatants was extracted by MagaBio Plus Viral DNA / RNA Purification Kit (BIOER). The reverse-transcription and analysis of RNA copies were performed using the HIV-1 Real-time PCR detection kit (BIOER).
Free full text: Click here
