Microstructural Analysis of Mouse Tibias

At the end of each experiment, the tibias of the mice were fixed in 4% paraformaldehyde. Samples were scanned using micro-CT (μCT 80; Scanco, Zurich, Switzerland) as previously described (38 (link)). The micro-CT parameters were as follows: voltage, 70 kV; electric current, 114 μA; and resolution, 10 μm per pixel. Three-dimensional structural parameters, including bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp), were analyzed (38 (link), 39 (link)).

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Du J., He Z., Xu M., Qu X., Cui J., Zhang S., Zhang S., Li H, & Yu Z. (2022). Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure. Frontiers in Endocrinology, 12, 778019.

Publication 2021

μCT 80

Bone Electric current Mice Micro ct Paraformaldehyde Tibias Trabecular

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : Peking University, Peking University Third Hospital, Peking University People's Hospital, Renji Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Bone volume fraction (BV/TV)
Trabecular number (Tb.N)
Trabecular thickness (Tb.Th)
Trabecular separation (Tb.Sp)

control variables

Voltage: 70 kV
Electric current: 114 μA
Resolution: 10 μm per pixel

controls

None explicitly mentioned

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