Isolation and Culture of Omentum-Derived Mast Cells

Omentum-derived MCs were obtained from non-uremic patients undergoing abdominal surgery. The omentum samples were digested in 0.05% trypsin and 0.02% EDTA to isolate MCs.16 (link),29 (link) All MCs isolated from the omentum were then incubated in culture medium consisting of M199 (Gibco) medium supplemented with 10% fetal bovine serum (FBS) (Biological Industries), insulin-transferrin-selenium-sodium pyruvate (ITS-A) (Gibco), 100 μg/mL of streptomycin (MDBio, Inc.), 63.6 µg/mL (Sigma) of penicillin G, and 250 ng/mL of Fungizone (MDBio, Inc.).24 (link)

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Lee Y.C., Huang C.T., Cheng F.Y., Hung S.Y., Lin T.M., Tsai Y.C., Chen C.I., Wang H.K., Lin C.W., Liou H.H., Chang M.Y., Wang H.H, & Chiou Y.Y. (2019). The Clinical Implication of Vitamin D Nanomedicine for Peritoneal Dialysis-Related Peritoneal Damage. International Journal of Nanomedicine, 14, 9665-9675.

Publication 2019

FBS insulin-transferrin-selenium-sodium pyruvate (ITS-A) streptomycin Fungizone

Abdominal Biological Edta Fetal bovine serum Fungizone Insulin Omentum Patients Penicillin g Pyruvate Selenium Sodium Streptomycin Surgery Transferrin Trypsin

Corresponding Organization :

Other organizations : I-Shou University, National Cheng Kung University, Chinese Culture University, Colorectal Surgical Associates, Jen-Ai Hospital

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Variable analysis

independent variables

Isolation of MCs from the omentum using 0.05% trypsin and 0.02% EDTA

dependent variables

Characteristics and behavior of isolated omentum-derived MCs

control variables

Incubation of isolated MCs in culture medium consisting of M199 (Gibco) medium supplemented with 10% fetal bovine serum (FBS) (Biological Industries), insulin-transferrin-selenium-sodium pyruvate (ITS-A) (Gibco), 100 μg/mL of streptomycin (MDBio, Inc.), 63.6 µg/mL (Sigma) of penicillin G, and 250 ng/mL of Fungizone (MDBio, Inc.)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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