siRNA oligonucleotides targeted to human Sun1, Sun2, nesprin1, nesprin2, lamin A/C, and a control siRNA targeted to luciferase (GL2), as described (Wiggan et al., 2017 (link)), were obtained from Qiagen. siRNAs for individual targets produced similar phenotypes and were used interchangeably between experiments.
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Knockdown of Nuclear Envelope Proteins
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Corresponding Organization : Colorado State University
Other organizations : Tokyo Institute of Technology
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Variable analysis
independent variables
- SiRNA oligonucleotides targeted to human Sun1
- SiRNA oligonucleotides targeted to human Sun2
- SiRNA oligonucleotides targeted to human nesprin1
- SiRNA oligonucleotides targeted to human nesprin2
- SiRNA oligonucleotides targeted to human lamin A/C
- Control siRNA targeted to luciferase (GL2)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: siRNA oligonucleotides targeted to luciferase (GL2)
- Negative control: Not explicitly mentioned
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