Lipoprotein Separation and Triglyceride Quantification

Lipoproteins were separated from 2.5 μl of individual plasma samples by size exclusion chromatography using a Superose 6 PC 3.2/300 column (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Lipoproteins were eluted as a fraction appearing in the exclusion volume of the sepharose column that contained VLDL, then LDL, and lastly HDL. TG concentrations were calculated after integration of the individual chromatograms (50 (link), 51 (link)), generated by the enzymatic-colorimetric reaction with the respective following kits, TG GPO-PAP (Roche Diagnostics, Mannheim, Germany).

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Delbès A.S., Quiñones M., Gobet C., Castel J., Denis R.G., Berthelet J., Weger B.D., Challet E., Charpagne A., Metairon S., Piccand J., Kraus M., Rohde B.H., Bial J., Wilson E.M., Vedin L.L., Minniti M.E., Pedrelli M., Parini P., Gachon F, & Luquet S. (2023). Mice with humanized livers reveal the role of hepatocyte clocks in rhythmic behavior. Science Advances, 9(20), eadf2982.

Publication 2023

Superose 6 PC 3.2/300 column TG GPO-PAP

Colorimetric Diagnostics Enzymatic Lipoproteins Plasma Sepharose Size exclusion chromatography

Corresponding Organization : University of Queensland

Other organizations : Institut Cochin, Inserm, Epigénétique et Destin Cellulaire, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Eurofins (Germany), Yecuris (United States), Karolinska Institutet, Karolinska University Hospital

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Variable analysis

independent variables

Plasma sample volume (2.5 μl)

dependent variables

Triglyceride (TG) concentrations

control variables

Size exclusion chromatography using a Superose 6 PC 3.2/300 column
Enzymatic-colorimetric reaction with TG GPO-PAP kit (Roche Diagnostics, Mannheim, Germany)

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