Quantification of Juvenile Hormone III

HPLC-grade methanol, acetonitrile, juvenile hormone III, triphenylphosphine (TPP), 2,2′–dipyridyl disulfide (DPDS), citronellol and dichloromethane were obtained from Sigma-Aldrich (St. Louis, MO). Farnesoic acid (Echelon, Salt Lake City, UT), sodium sulfide nonahydrate (MP Biomedicals, Solon, OH), DBD-COCl (4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole) and AABD–SH (4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) were from TCI-America (Portland, OR). JHB₃ was a gift from Dr. Stephen Tobe and was synthesized from methyl farnesoate using m-chloroperbenzoic acid in dichloromethane [36] . Diagnostic ions used for identification of JHB III included m/z  = 300, 283, 265, 251 and 301 as reported by Yin et al. [37] .