Vaginal Cytology for Estrous Staging

Estrous cycle stage was determined daily from 9–10 am, prior to lights off, by obtaining vaginal smears as previously described (Satta et al., 2018 (link)). Vaginal smears were analyzed immediately by bright field microscopy using an EVOS® FL inverted microscope (Thermo Fisher Scientific).

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Chen H., Lu Y., Xiong R., Rosales C.I., Coles C., Hamada K., Asad N., Thatcher G.R, & Lasek A.W. (2022). Effect of a brain‐penetrant selective estrogen receptor degrader (SERD) on binge drinking in female mice. Alcoholism, Clinical and Experimental Research, 46(7), 1313-1320.

Publication 2022

EVOS® FL inverted microscope

Estrous cycle Lights Microscope Vaginal smears

Corresponding Organization : University of Illinois Chicago

Other organizations : Illinois College

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Variable analysis

independent variables

Estrous cycle stage

dependent variables

Vaginal smear analysis

control variables

Time of day for vaginal smear collection (9-10 am, prior to lights off)
Vaginal smear collection method (as previously described in Satta et al., 2018)
Vaginal smear analysis using bright field microscopy with EVOS® FL inverted microscope

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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