Mitochondrial Protein Expression Analysis

Western blotting was conducted as in the previous studies [19 (link), 20 (link)]. Primary antibodies used herein include those targeting ND6 (Abcam, ab81212), ND4 (Abcam, ab219822), ATP6 (Proteintech, 55313-1-AP), ND1 (Abcam, ab74257), CYB (Proteintech, 55090-1AP), CO2 (Abcam, ab110258), and VDAC (Proteintech, 10866-1-AP). As a secondary detection antibody, we employed the peroxidase AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) constructs, and an enhanced chemiluminescence (ECL) system (Millipore) was used for band detection, after which densitometric calculations were performed.

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Li K., Wu L., Liu J., Lin W., Qi Q, & Zhao T. (2020). Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. Journal of Diabetes Research, 2020, 2057187.

Publication 2020

ab81212 AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG ECL) system

Anti igg Antibodies Antibody Chemiluminescence Densitometric Goat Mouse Peroxidase Rabbit

Corresponding Organization : Second Affiliated Hospital of Soochow University

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Variable analysis

independent variables

Primary antibodies targeting ND6, ND4, ATP6, ND1, CYB, CO2, and VDAC

dependent variables

Protein expression levels of ND6, ND4, ATP6, ND1, CYB, CO2, and VDAC

control variables

Western blotting protocol as in the previous studies [19, 20]
Use of peroxidase AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG as secondary detection antibodies
Use of an enhanced chemiluminescence (ECL) system for band detection

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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