Exosome Isolation and Characterization

Exosomes were isolated from the serum (5 ml) and cell culture medium by using ExoQuick ULTRA (System Biosciences, United States) according to the manufacturer’s instructions. The morphology and diameter size distribution of exosomes were assessed by FEI Tecnai T20 transmission electron microscopy (TEM; FEI Company, United States) according to the previous study (Li et al., 2015 (link)). The number of exosomes was determined by applying the BCA protein assay (Thermo Fisher Scientific, United States) (Soares Martins et al., 2018 (link)).

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Yan W., Wang Y., Chen Y., Guo Y., Li Q, & Wei X. (2021). Exosomal miR-130b-3p Promotes Progression and Tubular Formation Through Targeting PTEN in Oral Squamous Cell Carcinoma. Frontiers in Cell and Developmental Biology, 9, 616306.

Publication 2021

ExoQuick ULTRA FEI Tecnai T20 BCA protein assay

Assay Cell Cell culture Culture medium Exosomes Protein Serum Transmission electron microscopy

Corresponding Organization :

Other organizations : Cangzhou Central Hospital

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Variable analysis

independent variables

Isolation method: ExoQuick ULTRA (System Biosciences, United States)

dependent variables

Morphology and diameter size distribution of exosomes (assessed by FEI Tecnai T20 transmission electron microscopy (TEM; FEI Company, United States))
Number of exosomes (determined by applying the BCA protein assay (Thermo Fisher Scientific, United States))

control variables

Serum (5 ml) and cell culture medium (unspecified volume)

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