PFGE and MLST of K. pneumoniae
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Corresponding Organization : Shenzhen Children's Hospital
Other organizations : Shenzhen University, University Town of Shenzhen
Variable analysis
- Xbal digestion (45U, 2 hours at 37°C)
- PFGE patterns of K. pneumoniae isolates
- Amplified products of internal portions of seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) in K. pneumoniae
- Sequence types (STs) of K. pneumoniae isolates
- CHEFDRIII apparatus (Bio-Rad Laboratories, Hercules, CA, USA) for PFGE
- PCR assay with specific primers to amplify internal portions of the seven housekeeping genes
- Sequencing of amplified products by private enterprise (Sangon Biotech-Shanghai, China)
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