Frozen Embryo Transfer Protocol

Patients received FET cycle were treated with GnRH-a and hormone replacement therapy (HRT) before embryos transfer. Leuprorelin Acetate Microspheres for Injection (Enantone, Takeda, Japan) was intramuscularly injected on the day 2 of menstruation cycle with a dose of 3.75 mg. Estradiol valerate (Bugale, Bayer, Germany) were administered with a started dose of 4–6 mg/d after 28 days. When the endometrial thickness reached 7 mm, intramuscular injection of progesterone oil (Xianju Pharmaceutical, Zhejiang, China) 40 mg/d was provided for luteal support, after 4 or 6 days, the cleavage-stage embryos or blastocysts were transfrred.

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Li J., Li X., Ding J., Zhao J., Chen J., Guan F., Deng H., Zhou M., Han Y., Xiao Z, & Yang J. (2023). Analysis of pregnancy outcomes in patients with recurrent implantation failure complicated with chronic endometritis. Frontiers in Cell and Developmental Biology, 11, 1088586.

Publication 2023

Blastocysts Cleavage Embryos Enantone Endometrial Estradiol valerate Gnrh Hormone replacement therapy Intramuscular injection Leuprorelin acetate Luteal Menstruation cycle Microspheres Patients Pharmaceutical Progesterone

Corresponding Organization : Renmin Hospital of Wuhan University

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Variable analysis

independent variables

Leuprorelin Acetate Microspheres for Injection (Enantone, Takeda, Japan) intramuscularly injected
Estradiol valerate (Bugale, Bayer, Germany) administered
Progesterone oil (Xianju Pharmaceutical, Zhejiang, China) intramuscularly injected

dependent variables

Embryo transfer

control variables

Patients received FET cycle were treated with GnRH-a and hormone replacement therapy (HRT) before embryos transfer
Leuprorelin Acetate Microspheres for Injection (Enantone, Takeda, Japan) was intramuscularly injected on the day 2 of menstruation cycle with a dose of 3.75 mg
Estradiol valerate (Bugale, Bayer, Germany) were administered with a started dose of 4–6 mg/d after 28 days
When the endometrial thickness reached 7 mm, intramuscular injection of progesterone oil (Xianju Pharmaceutical, Zhejiang, China) 40 mg/d was provided for luteal support
After 4 or 6 days, the cleavage-stage embryos or blastocysts were transferred

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