Adoptive Transfer of IRF4-Overexpressing T Cells

BALB/c splenic dendritic cells (DCs) were isolated by using the Pan
Dendritic Cell Isolation Kit (Miltenyi Biotec, San Diego, CA).
Irf4^−/− CD4⁺ T cells
were activated for 3 days with BALB/c splenic DCs and 100 IU IL‐2
(PeproTech, Rocky Hill, NJ), and then transduced with IRF4‐GFP or
GFP‐Ctrl retrovirus as previously described.^{8 (link)} Cells were cultured for 1 day after
transduction, and then adoptively transferred into Irf4^fl/flCd4‐Cre mice on day 1 post‐heart
transplantation.

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Zhang H., Wu J., Zou D., Xiao X., Yan H., Li X.C, & Chen W. (2018). Ablation of interferon regulatory factor 4 in T cells induces “memory” of transplant tolerance that is irreversible by immune checkpoint blockade. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(3), 884-893.

Publication 2018

PanDendritic Cell Isolation Kit IL‐2

Cell isolation Cells Dendritic cells Irf4 Mice Retrovirus Splenic

Corresponding Organization : Cornell University

Other organizations : Second Xiangya Hospital of Central South University, Central South University, Union Hospital, Huazhong University of Science and Technology

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Variable analysis

independent variables

Genetic manipulation of T cells (Irf4-/- vs. Irf4fl/flCd4-Cre)
Transduction of T cells with IRF4-GFP or GFP-Ctrl retrovirus

dependent variables

T cell activation and proliferation
T cell function in vivo after adoptive transfer into mice

control variables

BALB/c splenic dendritic cells
IL-2 (100 IU)

controls

Positive control: Irf4-/- CD4+ T cells transduced with IRF4-GFP retrovirus
Negative control: Irf4-/- CD4+ T cells transduced with GFP-Ctrl retrovirus

Annotations

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