Producing Plasmodium falciparum Sporozoites

For producing P. falciparum sporozoites, female A. gambiae (Keele strain) or A. stephensi (Liston strain) mosquitoes were fed through an artificial glass membrane feeder with a blood mix containing P. falciparum NF54 (catalog number MRA-1000; BEI Resources) gametocytes adjusted to a final gametocytemia of 0.3% as described previously (14 (link)). Sporozoites were isolated from P. falciparum-infected A. gambiae or A. stephensi mosquitoes, and cryopreserved sporozoites were shipped on dry ice from JHMRI to Yale University for AgSAP binding studies.

Free full text: Click here

Arora G., Sajid A., Chuang Y.M., Dong Y., Gupta A., Gambardella K., DePonte K., Almeras L., Dimopolous G, & Fikrig E. (2021). Immunomodulation by Mosquito Salivary Protein AgSAP Contributes to Early Host Infection by Plasmodium. mBio, 12(6), e03091-21.

Publication 2021

MRA-1000

Artificial membrane Blood Dry ice Female Mosquitoes Sporozoites Strain

Corresponding Organization : Yale University

Other organizations : Johns Hopkins University, Aix-Marseille Université, Institut de Recherche pour le Développement, Institut de Médecine Tropicale du Service de Santé des Armées, Méditerranée Infection Foundation

Top 5 similar protocols

Variable analysis

independent variables

Mosquito species: Anopheles gambiae (Keele strain) or Anopheles stephensi (Liston strain)
Plasmodium falciparum NF54 gametocyte concentration (adjusted to 0.3% final gametocytemia)

dependent variables

Production of P. falciparum sporozoites

control variables

Blood mix containing P. falciparum NF54 gametocytes
Artificial glass membrane feeder
Cryopreservation and shipping of P. falciparum sporozoites

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!