Transcriptome analysis of glucocorticoid and mineralocorticoid receptor-mediated effects

All bioinformatics analyses were performed using the software CLC genomic workbench 9.0 (Qiagen, Germantown, MD, USA). Low-quality reads (Q < 20) and read lengths <50 bp were discarded from the raw data. The remaining reads were mapped onto a rainbow trout reference genome (GCA_013265735.3) composed of 71.413 coding sequences using default parameters. Transcripts with absolute fold-change ≥2.0 and an FDR of <0.05 were considered differentially expressed transcripts (DETs) in silico. A comparison between the vehicle and DOC groups considered potential DETs regulated by DOC. Comparisons between the DOC and mifepristone plus DOC groups, as well as the DOC and eplerenone plus DOC groups, considered potential DETs regulated by DOC and mediated by the glucocorticoid and mineralocorticoid receptors, respectively. The identification of gene IDs and DAVID GO enrichment analysis of differentially expressed transcripts were performed using a previously published approximation [20 (link)].