H3K27ac HiChIPs from EBV-infected LCLs

H3K27ac HiChIPs from LCLs conditional for EBNA3A expression were performed as previously described^{45 (link)} using H3K27ac antibody (Abcam). Briefly, 1 × 10⁷ cells for each biological replicate were collected and cross-linked by 1% formaldehyde for 10 min. Chromatin was digested using MboI restriction enzyme (New England Biolabs). DNA ends were filled in with Biotin-14-dATP (Thermo Fisher) and other nucleotides and then ligated. After sonication, sheared chromatin was pre-cleared and 3-fold diluted as described in ChIP method and then incubated with 4ug anti-H3K27ac antibody at 4 °C for overnight. Chromatin-antibody complex was captured by Dynabead Protein-A bead, followed by capture with Streptavidin C-1 bead (Thermo Fisher). Libraries were generated using Tn5 followed by PCR. HiChIP samples were size selected by PAGE purification (300–700 bp). All libraries were sequenced on the Illumina NextSeq 500. Each sample has an average depth of ~20 million reads.