As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Corresponding Organization : Peter MacCallum Cancer Centre
Other organizations :
Taronga Conservation Society Australia, University of Sydney, Centenary Institute, University of Michigan–Ann Arbor, St. Jude Children's Research Hospital, Monash University, Monash Medical Centre, Westmead Institute for Medical Research, Royal Melbourne Hospital, Icahn School of Medicine at Mount Sinai, Victorian Infectious Diseases Reference Laboratory
HCoV (229E, NL63, HKU1, OC43) spike and nucleoproteins (Sino Biological)
SARS-CoV-2 spike trimers (kind gifts from Adam K. Wheatley)
HKU-1 spike trimers (kind gifts from Adam K. Wheatley)
SARS-CoV spike trimers (BPS Bioscience)
NL63 spike trimers (BPS Bioscience)
dependent variables
Not explicitly mentioned
control variables
Tetanus toxoid (Sigma-Aldrich)
Influenza hemagglutinin (H1Cal2009; Sino Biological)
SIV gp120 (Sino Biological)
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