Heartworm Antigen Assays Before and After Heat Treatment

All antigen testing was conducted using commercial assays according to manufacturers’ instructions, with the exception that samples were tested before and after heat treatment on each assay. For heat treatment, serum samples were placed in a heat block at 103°C for 10 minutes, the resultant coagulum centrifuged, and the supernatant used in each commercial assay. Test kits evaluated before and after heat treatment included enzyme linked immunosorbent assay (ELISA) in microtiter plate formats (DiroCHEK®, Synbiotics Corporation, Zoetis; PetChek® Heartworm PF Antigen Test, IDEXX Laboratories, Inc.), membrane bound ELISAs (SNAP® Feline Heartworm® Test, IDEXX Laboratories, Inc.), and lateral flow immunochromatographic tests (WITNESS® HW, Synbiotics Corporation, Zoetis). In addition, O.D. readings were obtained by spectrophotometry before and after heat treatment for one of the microtiter plate assays (PetChek® Heartworm PF Antigen Test, IDEXX Laboratories, Inc.) according to manufacturer’s directions.

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Little S.E., Raymond M.R., Thomas J.E., Gruntmeir J., Hostetler J.A., Meinkoth J.H, & Blagburn B.L. (2014). Heat treatment prior to testing allows detection of antigen of Dirofilaria immitis in feline serum. Parasites & Vectors, 7, 1.

Publication 2014

PetChek® Heartworm PF Antigen Test

Antigen Assays Elisas Feline Heartworm Immunochromatographic Membrane Serum Spectrophotometry Synbiotics

Corresponding Organization :

Other organizations : Oklahoma State University, Bayer (United States), Auburn University

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Variable analysis

independent variables

Heat treatment of serum samples

dependent variables

Antigen detection results of commercial assays (ELISA, membrane bound ELISA, and lateral flow immunochromatographic tests) before and after heat treatment
Optical density (O.D.) readings of one of the microtiter plate assays (PetChek® Heartworm PF Antigen Test) before and after heat treatment

control variables

Commercial assays used according to manufacturers' instructions
Serum samples tested in the commercial assays before and after heat treatment

controls

Positive and negative controls were not explicitly mentioned in the input protocol.

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