Isolation and Activation of Mouse T Cells
Corresponding Organization : Cold Spring Harbor Laboratory
Other organizations : Memorial Sloan Kettering Cancer Center, Rockefeller University, Cornell University, New York Harbor School, University Children's Hospital Tübingen, Princeton University, Howard Hughes Medical Institute
Variable analysis
- Retroviral transduction of T cells
- T cell function
- Mouse strain (B6.SJL-Ptrca/BoyAiTac mice, CD45.1 mice)
- T cell purification method (mouse Pan T cell Isolation Kit)
- T cell culture conditions (RPMI-1640 medium, 10% FBS, 10 mM HEPES, 2 mM l-glutamine, MEM non-essential amino acids, 55 μM β-mercaptoethanol, 1 mM sodium pyruvate, 100 IU ml^-1 recombinant human IL-2, mouse anti-CD3/28 Dynabeads)
- Retroviral transduction protocol (spinoculation of T cells with retroviral supernatant collected from Phoenix-ECO cells 24 h after initial T cell activation)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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