LOX-1 Knockout Mice Generation

LOX-1 KO mice with a C57/BL6J background were produced as described previously [20 (link)] and LOX-1 KO mice and control wild-type (WT) C57/BL6J strain mice were a kind gift from Dr. Tatsuya Sawamura (National Cerebral and Cardiovascular Center, Suita, Japan/ Department of Physiology, Shinshu University School of Medicine, Matsumoto, Japan). Male LOX-1 KO mice and WT mice were used at the age of 12 weeks. Mice were housed in a facility with a 12-hour/12-hour light/dark cycle and were given free access to water and standard rodent chow. The room was kept specific-pathogen-free. DOX HCl (Sigma Chemical Co.) was dissolved in saline and administered by intraperitoneal injection at a dose of 20 mg/kg. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH Publication, 8th Edition, 2011). The protocol was approved by the Institutional Animal Research Committee of Gifu University (Permit Number: 17−68).