IHC Staining and H-Score Calculation for Protein Expression

IHC staining and H-Score calculating were performed as our previous research [17 (link)]. The primary antibodies used in the assay were anti-RRAGB (1:300, 13,023–1-AP, Proteintech, USA), anti-p-mTOR (Ser2448) (1:200, YP0176, Immunoway, USA), anti-HIF1A (1:300, YT2133, Immunoway, USA), and anti-cleaved-caspase3 (1:100, YM3431, Immunoway, USA).

Free full text: Click here

Li X., Wang J., Lin W., Yuan Q., Lu Y., Wang H., Chen Y., Chen L., Dai P., Long H, & Li X. (2022). circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. Molecular Cancer, 21, 135.

Publication 2022

YP0176 YT2133 YM3431

Antibodies Assay Caspase3 Hif1a Mtor

Corresponding Organization :

Other organizations : Southern Medical University, Wannan Medical College, Nanfang Hospital

Top 5 similar protocols

Variable analysis

independent variables

Anti-RRAGB (1:300, 13,023-1-AP, Proteintech, USA)
Anti-p-mTOR (Ser2448) (1:200, YP0176, Immunoway, USA)
Anti-HIF1A (1:300, YT2133, Immunoway, USA)
Anti-cleaved-caspase3 (1:100, YM3431, Immunoway, USA)

dependent variables

IHC staining
H-Score

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!