Partial sciatic nerve ligation was performed in male NMRI mice under ketamine/xylazine (100/5 mg/kg, i.p.) anesthesia, as previously described [25 (link)]. Briefly, the ipsilateral common sciatic nerve was exposed high in the thigh region and one-third to one-half of the nerve thickness was tightly ligated using a siliconized silk suture (Ethilon 8-0, Ethicon, Somerville, NJ, USA). The wound was closed with a skin suture (Mersilk 4-0, Ethicon, Somerville, NJ, USA), and the animals were allowed to survive for 7 days, because significant mechanical hyperalgesia develops without postoperative pain component by this time point [20 (link)].
Partial Sciatic Nerve Ligation Model of Neuropathic Pain
Partial sciatic nerve ligation was performed in male NMRI mice under ketamine/xylazine (100/5 mg/kg, i.p.) anesthesia, as previously described [25 (link)]. Briefly, the ipsilateral common sciatic nerve was exposed high in the thigh region and one-third to one-half of the nerve thickness was tightly ligated using a siliconized silk suture (Ethilon 8-0, Ethicon, Somerville, NJ, USA). The wound was closed with a skin suture (Mersilk 4-0, Ethicon, Somerville, NJ, USA), and the animals were allowed to survive for 7 days, because significant mechanical hyperalgesia develops without postoperative pain component by this time point [20 (link)].
Corresponding Organization : University of Pecs
Other organizations : Semmelweis University, Budapest University of Technology and Economics
Variable analysis
- Partial sciatic nerve ligation
- Mechanical hyperalgesia
- Ketamine/xylazine anesthesia
- Animal model (male NMRI mice)
- Timing of assessment (7 days after surgery)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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