Quantifying Cell Elongation via Microscopy

Following the static or shear culture with or without EMPA, cells were fixed in situ with 1% paraformaldehyde in PBS for 20 min, rinsed with PBS and stained with a 4% crystal violet (BD Biosciences) solution for 10 min. Cell morphology was assessed through light microscopy (Leica DMIL microscope and Leica DC300 camera) images and quantified by computing the shape index of individual cells using Matlab scripts^{59 (link)}. The shape index (SI) is used as a metric of cell elongation where a perfectly round cell has a SI close to 1 and a straight elongated cell, a SI closer to 0.

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Campeau M.A, & Leask R.L. (2022). Empagliflozin mitigates endothelial inflammation and attenuates endoplasmic reticulum stress signaling caused by sustained glycocalyx disruption. Scientific Reports, 12, 12681.

Publication 2022

crystal violet DMIL microscope DC300 camera

Cell Cells shape Crystal violet Light microscopy Microscope Paraformaldehyde

Corresponding Organization :

Other organizations : McGill University

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Variable analysis

independent variables

Static or shear culture
With or without EMPA

dependent variables

Cell morphology
Shape index (SI) of individual cells

control variables

Cell fixation in 1% paraformaldehyde in PBS for 20 min
Rinsing with PBS
Staining with 4% crystal violet solution for 10 min

controls

No positive or negative controls are explicitly mentioned.

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