Plasmid Sources and Lipid Composition

HEPES, Kacetate, MgCl₂, sodium phosphate monobasic, sodium phosphate dibasic, choloroform, trifluoroethanol, Octyl-ß-D-glucopyranoside were bought from Sigma Aldrich. DOPC (1,2dioleyl-sn-glycero-3-phosphocholine), DOPE (1,2dioleyl-sn-glycero-3-phosphoethanolamine), N-methyl-PE, N,N-dimethyl-PE, liver PI, Rhodamine-DOPE and NBD-DOPE were purchased from Avanti Polar Lipids, Inc. CAV1-GFP plasmid was purchased from Sino Biological (catalog no. HG11440-ACG). The following plasmids were gifts: YFP-CG2254 and YFP-CG9186 from Dr. Mathias Beller; GFP-Plin 1, GFP-Plin 1N, GFP-Plin 1C, mcherry-Plin 2 and mcherry-Plin 3 from Dr. David Savage; EGFP-ACSL3 plasmid from Dr. Joachim Füllekrug^{57 (link)}; GFP-HPos from Prof. Albert Pol^{40 (link)}. Cells were obtained from American Type Culture Collection and no contamination for mycoplasma was detected.