Macrophages were differentiated from monocytes with macrophage colony stimulating factor (Peprotech).
15 (link),
16 (link) In vitro phagocytosis was measured by fluorescence microscopy or live cell imaging using the IncuCyte System (Sartorius).
15 (link),
16 (link) Target cells were coincubated with macrophages at a 2:1 target cell to macrophage ratio. Target cells were labeled with CFSE (Biolegend, microscopy) or with pHrodo (Sartorius, IncuCyte). CD19‐DE and Hu5F9‐IgG2σ antibodies were applied to a concentration of 10 µg/mL. Engulfed cells were microscopically counted and a phagocytic index calculated (number of engulfed target cells per 100 macrophages), or displayed as relative red object counts per image (IncuCyte) after normalization. The largest value was defined as 100%. Experiments were performed with macrophages from different donors, as indicated.
15 (link),
16 (link) In vitro phagocytosis was measured by fluorescence microscopy or live cell imaging using the IncuCyte System (Sartorius).
15 (link),
16 (link) Target cells were coincubated with macrophages at a 2:1 target cell to macrophage ratio. Target cells were labeled with CFSE (Biolegend, microscopy) or with pHrodo (Sartorius, IncuCyte). CD19‐DE and Hu5F9‐IgG2σ antibodies were applied to a concentration of 10 µg/mL. Engulfed cells were microscopically counted and a phagocytic index calculated (number of engulfed target cells per 100 macrophages), or displayed as relative red object counts per image (IncuCyte) after normalization. The largest value was defined as 100%. Experiments were performed with macrophages from different donors, as indicated.
