Wound-healing assays were carried out to measure the effect of drugs on the migratory phenotype of PCa cells, as previously described (49 (link)). Briefly, cells were seeded in 6-well plates (1×106 cells per well) and grown until they formed a confluent monolayer. The monolayers were scratched using a 200 µl pipette tip, wells were washed with PBS and images of the wound (0-time point) were captured using a Leica Microsystems microscope (Buffalo Grove, IL, USA). Growth media (CS-FBS) was returned to each culture and treatments were initiated. Change in wound width was captured after 48 h and cell migration (wound closure) was calculated by measuring the distance between 4–5 random points within the wound edges.