Lipid and Glucose Profile Analysis

Blood specimens were obtained after a 12 to 14-h fast (8:00 p.m.–9:30 a.m.) to reduce the influence of circadian variation. Total cholesterol (TC) and triglyceride (TG) concentrations were assessed by using standard enzyme methods. The high-density lipoprotein (HDL) cholesterol level was measured after precipitation of very-low-density lipoproteins and low-density lipoprotein (LDL) with phosphotungstic acid, and LDL was calculated using the Friedewald formula. Fasting glucose levels were enzymatically measured by the hexokinase method. A blood sample from every patient was drawn and centrifuged within 30 min. The serum samples were stored at −80°C, and high sensitivity C-reactive protein (hs-CRP) was determined using an immunoturbidity assay (Liatest; Stago, Asnieres-sur-Seine, France), with an interassay variability coefficient of variation of 6.25%. The above method proceeded in the same way as in our previous study (15 (link)).