Western Blot Antibody Validation

Western blotting assay was performed as previously described^{46 (link)}. The anti-NHERF1 was purchased from Sigma-Aldrich (HPA009672, St. Louis, MO) and Becton Dickinson Labware (#611161, Billerica, MA), respectively. Anti-HA (#561) was purchased from Medical & Biological Laboratories (Nagoya, Japan). Anti-c-Myc (#ab32072) and anti-β-catenin (#ab22656) were purchased from Abcam (Cambridge, UK). Anti-GAPDH (#5174), anti-β-catenin (#9581), and anti-TCF-1 (#2206) were purchased from Cell Signaling Technology (Danvers, MA). Anti-ACTN4 was purchased from Enzo Life Sciences (#ALX-210-356, Shanghai, China) and Santa Cruz Biotechnology (#sc-134236, Santa Cruz, CA), respectively. Anti-Ki67 (#zm-0166) and horse radish peroxidase-conjugated secondary antibodies were purchased from ZSGB-BIO (Beijing, China). Infrared fluorescent dyes-conjugated secondary antibodies were purchased from LI-COR Biosciences (Lincoln, NE). IWR-1-endo (#S7086) was purchased from Selleck (Houston, TX).

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Wang Q., Qin Q., Song R., Zhao C., Liu H., Yang Y., Gu S., Zhou D, & He J. (2018). NHERF1 inhibits beta-catenin-mediated proliferation of cervical cancer cells through suppression of alpha-actinin-4 expression. Cell Death & Disease, 9(6), 668.

Publication 2018

Anti-HA anti-β-catenin Anti-GAPDH anti-TCF-1 Anti-Ki67 (#zm-0166) horse radish peroxidase-conjugated secondary antibodies IWR-1-endo

Actn4 Antibodies Biological Fluorescent dyes Gapdh Horse radish peroxidase Iwr 1 endo Nherf1 Tcf 1 Western blotting assay β catenin

Corresponding Organization :

Other organizations : Capital Medical University

Top 5 similar protocols

Variable analysis

independent variables

IWR-1-endo

dependent variables

NHERF1 protein expression
HA protein expression
C-Myc protein expression
β-catenin protein expression
GAPDH protein expression
TCF-1 protein expression
ACTN4 protein expression
Ki67 protein expression

control variables

Experimental method (Western blotting assay)
Anti-NHERF1 antibody (Sigma-Aldrich HPA009672)
Anti-HA antibody (#561, Medical & Biological Laboratories)
Anti-c-Myc antibody (#ab32072, Abcam)
Anti-β-catenin antibodies (#ab22656, Abcam; #9581, Cell Signaling Technology)
Anti-GAPDH antibody (#5174, Cell Signaling Technology)
Anti-TCF-1 antibody (#2206, Cell Signaling Technology)
Anti-ACTN4 antibodies (Enzo Life Sciences #ALX-210-356; Santa Cruz Biotechnology #sc-134236)
Anti-Ki67 antibody (#zm-0166, ZSGB-BIO)
Horseradish peroxidase-conjugated secondary antibodies (ZSGB-BIO)
Infrared fluorescent dyes-conjugated secondary antibodies (LI-COR Biosciences)

Annotations

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