NOD/ShiLtJ Mouse Model for Type 1 Diabetes

Non-obese diabetic (NOD/ShiLtJ) mice, a polygenic model for T1D, were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). NOD/ShiLtJ female mice exhibit diabetes beginning at around 16 weeks of age, characterized by insulitis, a leukocytic infiltrate of the pancreatic islets^{23 (link)}. Plasma glucose levels were used to determine disease onset. All animals for both studies were housed and bred in pathogen-free micro-isolator cages at the animal facilities operated by The Jackson Laboratory West (Sacramento, CA.). This study was conducted by In Vivo Services at The Jackson Laboratory Sacramento facility, an OLAW-assured and AAALAC-accredited organization. This study was performed according to the Jackson Laboratory IACUC-approved protocol and in compliance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 1996 [Liposome study] and 2011 [HSA study]).