Phytochemical Profiling of Sinomenium acutum
Corresponding Organization :
Other organizations : Korea Institute of Oriental Medicine, Korea University of Science and Technology
Variable analysis
- Sinomenium acutum extract concentration (20 mg/mL)
- Identification and quantification of phytochemical components (higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E)
- Chromatographic separation using Acquity BEH C18 column (100 × 2.1 mm, 1.7 µm)
- Mobile phase: 0.1% formic acid in water and acetonitrile
- UHPLC gradient eluent condition
- MS/MS parameters
- UHPLC-MS/MS system (Dionex UltiMate 3000 coupled with Thermo Q-Exactive)
- Data acquisition and processing software (Xcalibur and TraceFinder 5.1)
- Higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E standards purchased from TargetMol (Wellesley Hills, MA, USA)
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