Protein Analysis of Kidney Biopsies for AKI

A panel of proteins selected for their potential involvement in AKI were analysed in the selected donor kidney biopsies by western blotting and included: peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1a), mitofusin 1 and 2 (MFN1 and MFN2), peroxisome proliferator-activated receptor gamma (PPARg), heat shock protein (Hsp70), hepatocyte growth factor (HGF), glutathione S-transferase alfa (GSTa), transforming growth factor beta (TGFb), thioredoxin (TRX), peroxiredoxin (PRDX3), signal transducer and activator of transcription (STAT1), dynamin-related protein (DRP1) and phospho-DRP1, platelet derived growth factor receptor (PDGFRa), motif chemokine receptor 1 (CX3CR1), gamma-glutamyltransferase (gGT), Insulin-like growth factor-binding protein (IGFBP), glutathione S-transferase (GSTp). Detailed methods can be found in the Supplementary Material.

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Neri F., Lo Faro M.L., Kaisar M., Tam K.H., Borak M., Lindeman J., Angelini A., Fedrigo M., Kers J., Hunter J, & Ploeg R. (2024). Renal biopsies from donors with acute kidney injury show different molecular patterns according to the post-transplant function. Scientific Reports, 14, 6643.

Publication 2024

Corresponding Organization : University of Oxford

Other organizations : Oxford University Hospitals NHS Trust, Leiden University Medical Center, University of Padua, Amsterdam University Medical Centers, University of Amsterdam

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Variable analysis

independent variables

Selection of proteins for their potential involvement in AKI

dependent variables

Protein expression levels of PGC1a, MFN1, MFN2, PPARg, Hsp70, HGF, GSTa, TGFb, TRX, PRDX3, STAT1, DRP1, phospho-DRP1, PDGFRa, CX3CR1, gGT, IGFBP, GSTp

control variables

The selected donor kidney biopsies

controls

No positive or negative controls were explicitly mentioned in the provided information.

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