Genetically Modified Mouse Models

Batf3^−/−, Irf8^−/−, and Zbtb46^GFP/+ mice have been described previously. Irf8 +32^−/−, Irf8 −50^−/−, and Irf8 +41^−/− mice were newly generated as described below. The following mice were purchased from Jackson Laboratories: R26^Cas9/+ mice (B6N.129(Cg)-Gt(ROSA)26Sor^{tm1.1(CAG-cas9*,-EGFP)Fezh}/J), CD45.1⁺ mice (B6.SJL-Ptprc^aPepc^b/BoyJ), Tcf3^gfp/+ mice (B6.129-Tcf3^tm1Mbu/J), and VavCre^tg mice (B6.Cg-Commd10^{Tg(Vav1-iCre)A2Kio}/J). Tcf3^−/− mice were a gift from B. Kee. All mice were on the C57BL6/J background except for Tcf3^−/− mice, which were on the FVB/NJ background. All mice were generated, bred, and maintained in the Washington University in St. Louis School of Medicine specific pathogen-free animal facility. Animals were housed in individually ventilated cages covered with autoclaved bedding and provided with nesting material for environmental enrichment. Up to five mice were housed per cage. Cages were changed once a week, and irradiated food and water in autoclaved bottles were provided ad libitum. Animal manipulation was performed using standard protective procedures, including filtered air exchange systems, chlorine-based disinfection, and personnel protective equipment including gloves, gowns, shoe covers, face masks, and head caps. All animal studies followed institutional guidelines with protocols approved by the Animal Studies Committee at Washington University in St. Louis.
Unless otherwise specified, experiments were performed with mice between 6 and 10 weeks of age. No differences were observed between male and female mice in any assays performed and so mice of both genders were used interchangeably throughout this study. Within individual experiments, mice used were age- and sex-matched whenever possible. When mice were tracked for tumor growth or survival after Salmonella enterica Typhimurium infection, the monitoring scientist was blinded as to the genotypes of the mice in the experiment.