Matrigel Invasion Assay for Cell Migration

Cell invasion ability was evaluated by using a BioCoat Matrigel (Becton Dickinson Biosciences, Bedford, MA, USA) and Millicell invasion chamber (Millipore Corporation, Bedford, MA, USA) [26 (link),27 (link)]. The Matrigel was first coated onto the membrane of the Millicell upper chamber with a pore size of 8 μm in a 24-well plate. Cells in 1% FBS medium were seeded into the upper chamber. The lower chamber will contained 10% FBS in medium to trap invading cells. The invasion ability was determined by observing the reverse side of the upper chamber after being fixed and stained with crystal violet.

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You G.R., Chang J.T., Li Y.L., Huang C.W., Tsai Y.L., Fan K.H., Kang C.J., Huang S.F., Chang P.H, & Cheng A.J. (2022). MYH9 Facilitates Cell Invasion and Radioresistance in Head and Neck Cancer via Modulation of Cellular ROS Levels by Activating the MAPK-Nrf2-GCLC Pathway. Cells, 11(18), 2855.

Publication 2022

BioCoat Matrigel Millicell invasion chamber

Cells Crystal violet Matrigel Membrane

Corresponding Organization : Chang Gung Memorial Hospital

Other organizations : Taipei City Hospital

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Variable analysis

independent variables

Presence of Matrigel coating on the membrane of the Millicell upper chamber

dependent variables

Cell invasion ability

control variables

Pore size of the Millicell upper chamber (8 μm)
Cell seeding in 1% FBS medium in the upper chamber
10% FBS medium in the lower chamber

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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