Autoantibodies were measured in pre-transplant serum used for crossmatch or in a serum sample obtained in the month before transplantation. Anti-cardiolipin or anti-B2GPI of IgG and IgM isotypes were evaluated using the BioPLex 2200 multiplex immunoassay system (Biorad, Hercules CA, USA). Antibody levels above 18 U/mL were considered positive (99th percentile of healthy population).
IgA aCL and aBGPI antibodies were quantified by enzyme-linked immunosorbent assays (ELISA) using IgA-aCL and IgA-aB2GPI QUANTA Lite (INOVA Diagnostics Inc., San Diego, CA, USA). Antibody levels above 20 U/mL were considered positive. Cutoff were established with the 99th percentile of a healthy population in our hospital and coincided with the cutoff suggested in the assay manufacturer's guidelines (25 (link)).
Mean levels of anticardiolipin antibodies were: IgG 1.9 ± 0.2 and IgM 1.7 ± 0.3 IU/ml. Antibodies anti-B2GPI mean levels were: IgG 2.0 ± 0.4 IU/ml and IgM 1.8 ± 0.4 IU/ml. No significant differences were observed between the percentage of patients who were positive for anti-cardiolipin or anti-B2GPI (IgG/IgM isotypes) vs. healthy people. No association was found between the presence of IgG/IgM aPL and post-transplant mortality.
IgA aCL and aBGPI antibodies were quantified by enzyme-linked immunosorbent assays (ELISA) using IgA-aCL and IgA-aB2GPI QUANTA Lite (INOVA Diagnostics Inc., San Diego, CA, USA). Antibody levels above 20 U/mL were considered positive. Cutoff were established with the 99th percentile of a healthy population in our hospital and coincided with the cutoff suggested in the assay manufacturer's guidelines (25 (link)).
Mean levels of anticardiolipin antibodies were: IgG 1.9 ± 0.2 and IgM 1.7 ± 0.3 IU/ml. Antibodies anti-B2GPI mean levels were: IgG 2.0 ± 0.4 IU/ml and IgM 1.8 ± 0.4 IU/ml. No significant differences were observed between the percentage of patients who were positive for anti-cardiolipin or anti-B2GPI (IgG/IgM isotypes) vs. healthy people. No association was found between the presence of IgG/IgM aPL and post-transplant mortality.
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