To prepare DNA origami, M13mp18 scaffold, short DNA staple strands, and capture staples were mixed in 1×TAE/Mg2+ buffer (5 mm Tris, 1 mM EDTA, and 12.5 mm magnesium acetate, pH 8.0). The mixture was heated to 95 °C for 5 min in a thermal cycler and then allowed to cool down to 25°C at a rate of 0.1 °C every 10 s. Excess staples were then removed by washing with 500 µL 1×TAE/Mg2+ buffer (5 mM Tris, 1 mM EDTA, and 3 mm magnesium acetate, pH 8.0) four times using the Amicon Ultra‐0.5 mL centrifugal filter (MWCO 100 kD).
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