Respiration was measured in freely moving mice using plethysmography chambers (EMKA Technologies, France) supplied with a 5% CO2 in air mixture (BOC Gas Supplies, United Kingdom) as described previously (17 (link)). Rate and depth of respiration were recorded and averaged over 5 min periods (except immediately after drug injection when the time period was approximately 3 min) and converted to minute volume (rate × tidal volume).
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