Cryogenic Storage of T. reesei Spores

T. reesei RUT-C30 (ATCC 56765) was received from ATTC on October 2013, spread on PDA plates and incubated until sporulation. Spores were harvested with 50 % glycerol solution then stored at −80^∘C. Spore solution concentration was 6e9 mL ⁻¹. Culture media are prepared according to [53 (link)] (case with 25 m M dipotassium phthalate) and supplemented with 12.5 g L ⁻¹ glucose. Feeding solutions (stoichiometric mix of carbon and nitrogen sources) were prepared according to [52 (link)].

Free full text: Click here

Pirayre A., Duval L., Blugeon C., Firmo C., Perrin S., Jourdier E., Margeot A, & Bidard F. (2020). Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30. BMC Genomics, 21, 885.

Publication 2020

RUT-C30

Carbon Culture media Glucose Glycerol Nitrogen Phthalate Spore

Corresponding Organization : IFP Énergies nouvelles

Other organizations : Institut de Biologie de l'École Normale Supérieure, Centre National de la Recherche Scientifique, École Normale Supérieure - PSL, Inserm, Université Paris Sciences et Lettres

Top 5 similar protocols

Variable analysis

independent variables

Dipotassium phthalate concentration (25 mM)
Glucose concentration (12.5 g L^-1)

dependent variables

Not explicitly mentioned

control variables

Spore solution concentration (6e9 mL^-1)
Culture media composition (as per [53])
Feeding solutions composition (as per [52])

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!