Comprehensive Metabolite Profiling Protocol

Sixty-two conventional and targeted metabolites were assayed as previously described [12 (link)]. Briefly, conventional metabolite levels (lactate, triacylglycerols, 3-hydroxybutyrate, glycerol, NEFA) were measured on a Unicel DxC 600 clinical analyser (Beckman Coulter, Brea, CA, USA). Targeted metabolomics assays for acylcarnitines and amino acids were performed by tandem MS with addition of known quantities of stable isotope-labelled internal standards on an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA, USA).

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Kadakia R., Nodzenski M., Talbot O., Kuang A., Bain J.R., Muehlbauer M.J., Stevens R.D., Ilkayeva O.R., O’Neal S.K., Lowe L.P., Metzger B.E., Newgard C.B., Scholtens D.M, & Lowe WL J.r. (2018). Maternal metabolites during pregnancy are associated with newborn outcomes and hyperinsulinaemia across ancestries. Diabetologia, 62(3), 473-484.

Publication 2018

Unicel DxC 600 Acquity TQD Triple Quadrupole system

3 hydroxybutyrate Acylcarnitines Amino acids Assays Glycerol Isotope Lactate Nefa Triacylglycerols

Corresponding Organization : Northwestern University

Other organizations : Duke University, Duke University Hospital, Duke Medical Center

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Variable analysis

independent variables

Metabolite assays (conventional and targeted)

dependent variables

Levels of metabolites such as lactate, triacylglycerols, 3-hydroxybutyrate, glycerol, NEFA, acylcarnitines, and amino acids

control variables

Use of known quantities of stable isotope-labelled internal standards

controls

Positive control: Known quantities of stable isotope-labelled internal standards added to the assays
Negative control: Not explicitly mentioned

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