Flies were raised at 25°C and 60% relative humidity on standard cornmeal fly food under 12:12h light:dark cycle. 4–7 days old flies were used for experiments and were transferred to fresh food vials 48h before behavioral tests. Fly population was randomized but was kept age matched in each trial. For food deprivation, flies were kept in empty bottles with a wet cotton plug to prevent desiccation. The following fly lines were ordered from Bloomington stock center: Npf-Gal4 (25681), npfr mutants (10747), 20XUAS-TTS-shi[ts1]-p10 (66600; referred to as UAS-shibirets1 in the text), UAS-TrpA1 (26263), MB461B (68327), MB370B (68319), MB504B (68329), MB296B (68308), MB320C (68253), MB077B (68283) and MB112C (68263). UAS-npf-RNAi (108772), UAS-npfr-RNAi (107663) and VT50658 (200166) were ordered from the Vienna Drosophila Resource Center. Other fly lines were described previously: redeye6 (link), R26E0115 (link), R35B1216 , and UAS-CaLexA17 (link). The background control line was Canton-S (Heisenberg) strain.
Protocol detail
Fly Behavioral Assay Protocols
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Temperature (25°C)
- Relative humidity (60%)
- Light-dark cycle (12:12h)
- Food deprivation
- Genotypes of fly lines (Npf-Gal4, npfr mutants, UAS-shibire^(ts1), UAS-TrpA1, etc.)
- Fly behavior during behavioral tests
- Fly age (4-7 days old)
- Fly food (standard cornmeal fly food)
- Fly population randomization (age-matched within each trial)
- Canton-S (Heisenberg) strain as background control line
- UAS-shibire^(ts1)
- UAS-TrpA1
- Canton-S (Heisenberg) strain
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!