Western blot analysis was performed as described (Izumchenko et al, 2009 (link)). Anti-Abcc10 antibody was used at a concentration of 1 : 500 (Hopper-Borge et al, 2011 (link)). ABCB1, ABCG2 (Santa Cruz Biotechnology, Dallas, TX, USA), β-actin (Abcam, Cambridge, MA, USA), HER2 and ERα (Cell Signalling Technology, Danvers, MA, USA) antibodies were used according to the manufacturer's instructions.
Protocol detail
Western Blot Analysis of ABC Transporters
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Corresponding Organization :
Other organizations : Fox Chase Cancer Center
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Variable analysis
independent variables
- Anti-Abcc10 antibody concentration (1 : 500)
- Abcc10 protein expression
- ABCB1 protein expression
- ABCG2 protein expression
- β-actin protein expression
- HER2 protein expression
- ERα protein expression
- Positive control: Not specified
- Negative control: Not specified
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