Collagenase and autologous blood injection ICH models were performed as described previously.3 (link), 10 (link), 17 (link) Briefly, under isoflurane anesthesia, 0.5 μl of 0.2 U type VII-S collagenase (Sigma-Aldrich) or 25 μl autologous blood was injected into the right striatum at the following coordinates relative to bregma: 2.5 mm lateral, and 3.0 mm deep at 5 ° angle. The rectal temperature was maintained at 37.0 ± 0.5 °C throughout the surgery and recovery periods.
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Collagenase and Autologous Blood ICH Models
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Other organizations : National Taiwan University, Yale University, Universidade de São Paulo
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Variable analysis
independent variables
- Type of injection (Collagenase or autologous blood)
- Not explicitly mentioned
- Isoflurane anesthesia
- Injection location (right striatum at 2.5 mm lateral, 3.0 mm deep at 5° angle relative to bregma)
- Rectal temperature maintained at 37.0 ± 0.5 °C
- No positive or negative controls were explicitly mentioned.
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