Embryos were fixed in 4% formaldehyde, phosphate buffer pH 7.4 as previously described [45 (link)]. The primary antibodies used for indirect immunofluorescence were as follows: guinea pig anti PATJ (1 : 500, [30 (link)]), mouse anti Sdt (1 : 20, [42 (link)]), rabbit anti Sdt (1 : 2000, this study), rabbit anti Baz (1 : 1000, [43 (link)]), mouse anti Crb (Cq4, 1 : 50, DSHB), mouse anti Dlg (1 : 50, DSHB), rabbit anti GFP (#A11122, 1 : 1000, Life Technologies) and chicken anti GFP (1 : 2000, Aves Laboratories). Secondary antibodies conjugated with Alexa 488, Alexa 568 and Alexa 647 (Life Technologies) were used at 1 : 400.
Images were taken on a Zeiss LSM 710 Meta confocal microscope and processed using Adobe Photoshop.
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