Immunohistochemical Analysis of Kidney Tissue
Corresponding Organization : University of Sydney
Other organizations : University of Technology Sydney
Protocol cited in 1 other protocol
Variable analysis
- Antigen retrieval temperature (99 °C)
- Antigen retrieval duration (20 min)
- Staining area percentage of Fibronectin
- Staining area percentage of Collagen type I
- Staining area percentage of 8-hydroxy-2′ -deoxyguanosine (8-OHdg)
- Tissue fixation (10% formalin for 36-h)
- Tissue embedding (paraffin or frozen-embedded in OCT solution)
- Tissue sectioning (4-μm thickness)
- Peroxidase deactivation (3% H2O2)
- Protein blocking (Protein Block Serum-Free)
- Primary antibody incubation (overnight at 4 °C)
- Secondary antibody incubation (30 mins)
- HRP-conjugated streptavidin incubation (10 mins)
- Image acquisition (Leica DM750 photomicroscope, 20× magnification)
- Image analysis (ImageJ software)
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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