Muscle Larvae SiRNA Knockdown Assay

In 12-well culture plates (500 ML/500 μL) at 37 °C with 5% CO₂, muscle larvae were cultured in RPMI-1640 (supplemented with penicillin 500 units/mL and streptomycin 500 mg/mL) with siRNA-180, siRNA-419, siRNA-559, siRNA-Control, and PBS control as described above [42 (link)]. ML stage parasites were tested for motility and ecdysis (molting process). An inverted bright field microscope (Olympus, Shibuya, Japan) was used to examine the phenotypic appearance [24 (link)].

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Aleem M.T., Wen Z., Yu Z., Chen C., Lu M., Xu L., Song X., Li X, & Yan R. (2023). Inhibition of Trichinella spiralis Membrane-Associated Progesterone Receptor (MAPR) Results in a Reduction in Worm Burden. Vaccines, 11(9), 1437.

Publication 2023

inverted bright field microscope

Larvae Microscope Motility Muscle Parasites Penicillin Phenotypic Sirna Streptomycin

Corresponding Organization : Nanjing Agricultural University

Other organizations : Cleveland State University, Ningxia University

Top 5 similar protocols

Variable analysis

independent variables

SiRNA-180
SiRNA-419
SiRNA-559
SiRNA-Control
PBS control

dependent variables

Motility
Ecdysis (molting process)

control variables

12-well culture plates (500 ML/500 μL)
Temperature at 37 °C
5% CO2
RPMI-1640 (supplemented with penicillin 500 units/mL and streptomycin 500 mg/mL)

controls

SiRNA-Control
PBS control

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