The wound contraction rate was assessed with the help of a transparent paper over the wound site and tracing it52 ,53 (link). The total bacterial count was also measured with the help of plate count agar (Merck KGaA, Darmstadt, Germany). In summary, 0.1 g of the wound sample was crushed, minced, and diluted and cultured on plate count agar. The cultured plates were incubated at 37 °C for 48 h and the total bacterial count was calculated.
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