Total RNA was extracted with TRIzol Reagent (Invitrogen), followed by cDNA synthesis using a TransScript All-in-One First-Strand cDNA Synthesis SuperMix (Transgen Biotech, Beijing, China), was performed as described previously [24 (link)]. PCR was performed using a Bio-Rad PCR instrument (Bio-Rad, Hercules, CA, U.S.A.) with 2× Taq PCR Master Mix (Solarbio, Beijing, China) following the manufacturer’s instructions. The fold changes were calculated by means of relative quantification (2−△△Ct method). PCR primers are described as below:
MALAT1: forward 5′-GGGTGTTTACGTAGACCAGAACC-3′ and reverse 5′-CTTCCAAAAGCCTTCTGCCTTAG-3′; miR-124: forward 5′-TCGTTAAGGCACGCGGTG-3′ and reverse 5′-GTGCAGGGTCCGAGGT-3′; U6: forward 5′-CTCGCTTCGCAGCACA-3′ and reverse 5′-AACGCTTCACGAATTTGCGT-3′; GAPDH forward 5′-GCACCGTCAAGGCTGAGAAC-3′ and reverse 5′-ATGGTGGTGAAGACGCCAGT-3′.
MALAT1: forward 5′-GGGTGTTTACGTAGACCAGAACC-3′ and reverse 5′-CTTCCAAAAGCCTTCTGCCTTAG-3′; miR-124: forward 5′-TCGTTAAGGCACGCGGTG-3′ and reverse 5′-GTGCAGGGTCCGAGGT-3′; U6: forward 5′-CTCGCTTCGCAGCACA-3′ and reverse 5′-AACGCTTCACGAATTTGCGT-3′; GAPDH forward 5′-GCACCGTCAAGGCTGAGAAC-3′ and reverse 5′-ATGGTGGTGAAGACGCCAGT-3′.
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