Sensitive miR-362-3p Detection in RCC

A 5′-DIG and 3′-DIG labeled, locked nucleic acid-incorporated miRNA probe (miRCURY LNATM Detection probe, Exiqon, Woburn, MA, United States) was used for the detection of miR-362-3p in RCC TMA. The probe sequence was designed as follows: 5′–3′. The specific manipulations were performed as previously described (Liang et al., 2017 (link)).

Free full text: Click here

Zhu H., Wang S., Shen H., Zheng X, & Xu X. (2020). SP1/AKT/FOXO3 Signaling Is Involved in miR-362-3p-Mediated Inhibition of Cell-Cycle Pathway and EMT Progression in Renal Cell Carcinoma. Frontiers in Cell and Developmental Biology, 8, 297.

Publication 2020

miRCURY LNATM Detection probe

Mirna Nucleic acid probe

Corresponding Organization : Zhejiang University

Top 5 similar protocols

Variable analysis

independent variables

Use of a 5'-DIG and 3'-DIG labeled, locked nucleic acid-incorporated miRNA probe (miRCURY LNATM Detection probe, Exiqon, Woburn, MA, United States)

dependent variables

Detection of miR-362-3p in RCC TMA

control variables

Specific manipulations were performed as previously described (Liang et al., 2017)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!