Cells were treated with mibefradil (0.01–1 µM; Sigma Aldrich) [33 (link)] or ML218 (0.1–10 µM; Sigma Aldrich) [34 (link)] either alone or in combination with trastuzumab (10 µg/mL), 24 h after seeding (2000 cells/well in a 96 well plate). All treatments and media changes were repeated every 2 days in antibiotic-free media. Sensitivity to trastuzumab was assessed using a MTS assay 192 h after treatment start.
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